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Image Search Results
Journal: Frontiers in Molecular Neuroscience
Article Title: MicroRNA-133b Negatively Regulates Zebrafish Single Mauthner-Cell Axon Regeneration through Targeting tppp3 in Vivo
doi: 10.3389/fnmol.2017.00375
Figure Lengend Snippet: miR-shRNA based gene silence of tppp3 diminishes regenerative length of M-cell (A) Diagram of miR-shRNA system. Sequence of ShRNA-5 was presented here, with the guide strand (bottom) highlighted in dark blue. (B) Quantitative RT-PCR analysis exhibited a deduction of tppp3 mRNA in shRNA-5 expressing embryos. (C) Confocal imaging of M-cell at 2 dpa. White asterisk: ablation point. Scale bar: 50 μm. (D) Regeneration length at 2 dpa. Student's two-tailed t -test, control vs. shRNA-1, P = 0.5807; control vs. shRNA-5, P = 0.0025. ** P < 0.01. Error bars represent S.E.M.
Article Snippet: Based on the Web-based
Techniques: shRNA, Sequencing, Quantitative RT-PCR, Expressing, Imaging, Two Tailed Test